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From July — September , our most downloaded Analytical Methods articles were:. Principal component analysis Rasmus Bro and Age K. Smilde Anal. Methods , , 6 , DOI:
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Ubiquitin-specific proteases USPs reverse ubiquitination and regulate virtually all cellular processes. No such interactions have been reported for these domains in the paralog USP11, a key regulator of DNA double-strand break repair by homologous recombination. We hypothesized that USP11 domains adjacent to its protease domain harbor unique peptide-binding sites. Here, using a next-generation phage display NGPD strategy, combining phage display library screening with next-generation sequencing, we discovered unique USPinteracting peptide motifs. The highest affinity USP11 peptide binder fused to a cellular delivery sequence induced significant nuclear localization and cell cycle arrest in S phase, affecting the viability of different mammalian cell lines.
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